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APC Anti-Mouse CD86 (B7-2) (GL-1)

Sizes available: 25 µg, 100 µg

Applications: FC
SKU: 20-0862
Product Name Price Qty
APC Anti-Mouse CD86 (B7-2) (GL-1) ..... Cat.No. 20-0862-U025 ..... Size 25 µg
$51.00
APC Anti-Mouse CD86 (B7-2) (GL-1) ..... Cat.No. 20-0862-U100 ..... Size 100 µg
$151.00

Description

Details

The GL-1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Additional Info

Additional Info

Name APC Anti-Mouse CD86 (B7-2) (GL-1)
Cat. No. 20-0862
Technical Data Sheet Download TDS
Alternative Names B7.2, B70, Ly-58
Gene ID 12524
Clone GL-1 (GL1)
Isotype Rat IgG2a, kappa
Reactivity Mouse
Cross Reactivity
Format APC
Applications Flow Cytometry
Citations*

Liu Z, Geboes K, Hellings P, Maerten P, Heremans H, Vandenberghe P, Boon L, van Kooten P, Rutgeerts P, and Ceuppens JL. 2011. J. Immunol. 167: 1830-1838. (in vivo blocking, Immunohistochemistry – OCT embedded frozen tissue)

Kastenmuller W, Gasteiger G, Subramanian N, Sparwasser T, Busch DH, Belkaid Y, Drexler I, and Germain RN, 2011. J. Immunol. 187: 3186-3197. (in vivo blocking)

Zheng SG, Wang JH, Stohl, W, Kim KS, Gray JD, and Horwitz DA. 2006. J. Immunol. 176:3321-3329. (in vitro blocking)

Leithauser F, Meinhardt-Krajina T, Fink K, Wotschke B, Moller P and Reimann J. 2006. Am. J. Pathol. 168(6): 1898-1909. (Immunohistochemistry – frozen tissue)

Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy – frozen tissue, Immunohistochemistry – frozen tissue)

Lenschow DJ, Ho SC, Sattar H, Rhee L, Gray G, Nabavi N,Herold KC, and Bluestone JA. 1995. J. Exp. Med. 181:1145-155. (in vitro blocking)

Blazar BR, Taylor PA, Panoskaltsis-Mortari A, Gray GS, and Vallera DA. 1995. Blood. 85: 2607-2618. (Immunohistochemistry – OCT embedded frozen tissue)

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Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.