The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.
The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3. Please choose the appropriate format for each application.
|Name||APC Anti-Human CD3 (Hit3a)|
|Technical Data Sheet||Download TDS|
|Alternative Names||Leu-4, T3|
|Isotype||Mouse IgG2a, kappa|
Lesourne R, Zvezdova E, Song K-D, El-Khoury D, Uehara S, Barr VA, Samelson LE and Love PE. 2012. J. Immunol. 189: 1154-1161. (in vitro activation)
Knyazhitsky M, Moas E, Shaginov E, Luria A, and Braiman A. 2012. J. Biol. Chem. 287: 19725-19735. (in vitro activation)
Ge Shuwang, Hertel B, Emden SH, Beneke J, Menne J, Haller H, and von Vietinghoff S. 2012. Nephrol. Dial. Transplant. 27: 2768-2772. (Immunofluorescence microscopy)
Soto PC, Stein LL, Hurtado-Ziola N, Hedrick SM, and Varki A. 2010. J. Immunol. 184: 4185-4195. (Flow cytometry – Chimpanzee)
Westermann J, Bode U, Sahle A, Speck U, Karin N, Bell EB, Kalies K, and Gebert A. 2005. J. Immunol. 174: 2517-2524. (Immunohistochemistry – frozen tissue)
Mukouyama H, Janzen NK, Hernandez JM, Lam JS, Caliliw R, Wang AY, Figlin RA, Belldegrun AS, and Zeng G. 2004. Clin. Cancer Res. 10: 1421-1429. (in vitro blocking)