Toll-Free Customer Service (855) 848-6626 Hours 6AM-5PM PST

FREE Shipping on orders over $300*

Your valued source for Immunology Tools

Recombinant Human TNF-α (Carrier-free)

Sizes available: 10 µg, 50 µg, 100 µg, 500 µg

Applications: Bioassay

SKU: 21-8329
Recombinant Human TNF-α (Carrier-free) is available for purchase in increments of 1
Product Name Price Qty
Recombinant Human TNF-α (Carrier-free) ….. Cat.No. 21-8329-U010 ….. Size 10 µg
$125.00
Recombinant Human TNF-α (Carrier-free) ….. Cat.No. 21-8329-U050 ….. Size 50 µg
$200.00
Recombinant Human TNF-α (Carrier-free) ….. Cat.No. 21-8329-U100 ….. Size 100 µg
$342.00
Recombinant Human TNF-α (Carrier-free) ….. Cat.No. 21-8329-U500 ….. Size 500 µg
$798.00

Description

Details

TNF-alpha is a clinically important cytokine with strong pro-inflammatory activities. TNF-alpha is secreted by a variety of immune and non-immune cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. TNF-alpha signals through two receptors, TNFR1 and TNFR2. TNF-alpha has strong cytolytic activities against a wide variety of tumor cells and is critically important in combat against bacterial infections. The inflammatory effects of TNF-alpha are also deleterious in causation of septic shock, and autoimmune diseases including rheumatoid arthritis, inflammation, and diabetes.

Additional Info

Additional Info

Name Recombinant Human TNF-α (Carrier-free)
Cat. No. 21-8329
Technical Data Sheet Download TDS
Alternative Names Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin
Amino Acid Sequence VRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity ED50 is determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human TNF alpha is a soluble 157 amino acid protein of 17.4 kDa which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas AIDS/HIV; Angiogenesis/Cardiovascular; Apoptosis; Cancer; Diabetes / Weight Regulation; Immune System; Inflammation; Neurobiology; Stem Cells & Differentiation; TNF Superfamily; Allergy; Transplantation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Applications Bioassay
Need Another Format? Inquire Here

Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.