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Recombinant Human sCD40 Ligand (Carrier-free)

Sizes available: 10 µg, 50 µg, 500 µg
Applications: Bioassay
SKU: 21-7088
Recombinant Human sCD40 Ligand (Carrier-free) is available for purchase in increments of 1
Product Name Price Qty
Recombinant Human sCD40 Ligand (Carrier-free) ..... Cat.No. 21-7088-U010 ..... Size 10 µg
$69.00
Recombinant Human sCD40 Ligand (Carrier-free) ..... Cat.No. 21-7088-U050 ..... Size 50 µg
$418.00
Recombinant Human sCD40 Ligand (Carrier-free) ..... Cat.No. 21-7088-U500 ..... Size 500 µg
$829.00

Description

Details

CD40 Ligand (CD40L, CD154, TRAP, TNFSF5, gp39) belongs to the TNF superfamily and in its soluble form, displays some cytokine-like properties. CD40L binds to its receptor, CD40, to mediate T cell-dependent B cell responses and contribute to monocyte activation and dendritic cell maturation. Membrane-bound CD40L is expressed mainly on activated T cells, a small population of CD8+ T cells, and has been demonstrated on mast cells, basophils, eosinophils, B cells, NK cells and monocytes/macrophages. Activated platelets express CD40L on their surface and constitute the major source of soluble CD40L (sCD40L). sCD40L is a shorter protein than the membrane-bound version and retains biologic activity and ability to form trimers. CD40L expression can be upregulated using mitogens like PMA or PHA, through anti-CD3 activation, or by thrombin in platelets.

Additional Info

Additional Info

Name Recombinant Human sCD40 Ligand (Carrier-free)
Cat. No. 21-7088
Technical Data Sheet Download TDS
Alternative Names soluble CD40 Ligand, TNFSF5, TRAP, CD154, gp39, T-BAM
Amino Acid Sequence MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Assay #1: Determined by the stimulation of IL-12 induction by human PBMCs.
Assay #2: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Angiogenesis/Cardiovascular; Apoptosis; Immune System; Inflammation; Proliferation; TNF Superfamily; Allergy; Transplantation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Applications Bioassay
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Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.