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Recombinant Human LIGHT (Carrier-free)

Sizes available: 3 µg, 15 µg
Applications: Bioassay
SKU: 21-7155
Recombinant Human LIGHT (Carrier-free) is available for purchase in increments of 1
Product Name Price Qty
Recombinant Human LIGHT (Carrier-free) ..... Cat.No. 21-7155-U003 ..... Size 3 µg
$76.00
Recombinant Human LIGHT (Carrier-free) ..... Cat.No. 21-7155-U015 ..... Size 15 µg
$175.00

Description

Details

LIGHT, also known as TNFSF14, CD258 and HVEM-L, is a type II transmembrane protein belonging to the TNF family of ligands. It is produced by activated T-cells and signals via the lymphotoxin beta receptor (LTbetaR) and the herpes virus entry mediator (HVEM). It is a potent initiator of T cell co-stimulation signals that play a role in tumor and allograft rejection. LIGHT is reported to work together with IFN-g to activate caspase activity, resulting in cleavage of Bcl-2.

Additional Info

Additional Info

Name Recombinant Human LIGHT (Carrier-free)
Cat. No. 21-7155
Technical Data Sheet Download TDS
Alternative Names TNFSF14, HVEM-L, CD258
Amino Acid Sequence RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Its cytotoxic effect, in the presence of human IFN-g, on human colon adenocarcinoma cells (HT-29) is determined.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human LIGHT consists of 177 amino acids and has a calculated molecular weight of 19.3 kDa. By SDS-PAGE, LIGHT migrates between 20.0-22.5 kDa under non-reducing conditions due to glycosylation.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source Hi-5 Insect cells
Reactivity Human
Research Areas TNF Superfamily, Immune System, Apoptosis, Cancer, Inflammation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
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Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHCF = Immunohistochemistry, Frozen Tissue; IHCP = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.