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Recombinant Human sCD14 (Carrier-free)

Sizes available: 10 µg, 50 µg, 100 µg, 500 µg
Applications: Bioassay
SKU: 21-7173
Recombinant Human sCD14 (Carrier-free) is available for purchase in increments of 1
Product Name Price Qty
Recombinant Human sCD14 (Carrier-free) ..... Cat.No. 21-7173-U010 ..... Size 10 µg
$69.00
Recombinant Human sCD14 (Carrier-free) ..... Cat.No. 21-7173-U050 ..... Size 50 µg
$175.00
Recombinant Human sCD14 (Carrier-free) ..... Cat.No. 21-7173-U100 ..... Size 100 µg
$280.00
Recombinant Human sCD14 (Carrier-free) ..... Cat.No. 21-7173-U500 ..... Size 500 µg
$859.00

Description

Details

CD14, expressed mainly by monocytes and macrophages, is generally present as a membrane-bound surface glycoprotein. It serves as a homing receptor for lipopolysaccharide (LPS) and is a pattern recognition receptor for a variety of microbial-derived ligands. CD14 is not a signaling protein and forms a receptor complex with toll-like receptor 4 (TLR4) to transduce CD14-LPS signals. CD14 is also present as a soluble format in serum and other bodily fluids (sCD14). This soluble format can be shed from the cell surface and may play a role in inhibiting LPS-mediated responses in extracellular spaces.

Additional Info

Additional Info

Name Recombinant Human sCD14 (Carrier-free)
Cat. No. 21-7173
Technical Data Sheet Download TDS
Alternative Names soluble CD14, Monocyte Differentiation Antigen CD14
Amino Acid Sequence TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QP
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Using a concentration range of 20 - 200 ng/ul, dose dependent activation of NF-kB in a RAW264 cell line based reporter system is measured. In the presence of 0.25 - 1.0 ng/ul of bacterial LPS enhances the activation assay.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human sCD14 is a 35.6 kDa protein comprising 331 amino acids of the extracellular portion of the CD14 receptor.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source HEK293 cells
Reactivity Human
Research Areas Receptors, Inflammation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Applications Bioassay
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Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.