Description
Details
The SK3 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.
The SK3 antibody is widely used as a phenotypic marker for human CD4 expression, and has been reported to be cross-reactive with Rhesus and Cynomolgus CD4. This antibody does not block binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.
Additional Info
Additional Info
Name | PerCP-Cyanine5.5 Anti-Human CD4 (SK3) |
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Cat. No. | 65-0047 |
Technical Data Sheet | Download TDS |
Alternative Names | Leu-3, T4 |
Gene ID | 920 |
Clone | SK3 |
Isotype | Mouse IgG1, kappa |
Reactivity | Human |
Cross Reactivity | Cynomolgus, Rhesus |
Format | PerCP-Cyanine5.5 |
Application | Flow Cytometry |
Citations* | Evans RL, Wall DW, Platsoucas CD, Siegal FP, Fikrig SM, Testa CM and Good RA. 1981. Proc Natl Acad Sci U S A. 78(1): 544-548. Sattentau QJ, Dalgleish AG, Weiss RA and Beverley PC. 1986. Science. 234(4780): 1120-1123. Bernard A, Boumsell L and Hill C. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984: 9-108. (Flow Cytometry) Heninger AK, Theil A, Wilhelm C, Petzold C, Huebel N, Kretschmer K, Bonifacio E and Monti P. 2012. J Immunol. 189(12): 5649-5648. (Flow Cytometry) Yoshino N, Ami Y, Terao K, Tashiro F and Honda M. 2000. Exp. Anim. 49(2): 97-110. (Flow Cytometry - Cynomolgus) Lafont BAP, Gloeckler L, D'Hautcourt JL, Gut JP and Aubertin AM. 2000. Cytometry 41: 193-202. (Flow Cytometry - Rhesus) |