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PE-Cyanine7 Anti-Mouse TCR beta (H57-597)

Sizes available: 25µg, 100µg

Applications: FC
SKU: 60-5961
PE-Cyanine7 Anti-Mouse TCR beta (H57-597) is available for purchase in increments of 1
Product Name Price Qty
PE-Cyanine7 Anti-Mouse TCR beta (H57-597) ..... Cat.No. 60-5961-U025 ..... Size 25 µg
$75.00
PE-Cyanine7 Anti-Mouse TCR beta (H57-597) ..... Cat.No. 60-5961-U100 ..... Size 100 µg
$190.00

Description

Details

The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma/delta protein chains, known as the g/d TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.

The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the g/d TCR.

Additional Info

Additional Info

Name PE-Cyanine7 Anti-Mouse TCR beta (H57-597)
Cat. No. 60-5961
Technical Data Sheet Download TDS
Alternative Names TCRb, TCRbeta, TCR-b chain, TCR-b, b-TCR
Gene ID 21577
Clone H57-597
Isotype Armenian Hamster IgG
Reactivity Mouse
Cross Reactivity No
Format PE-Cyanine7
Applications Flow Cytometry
Citations*

Berent-Maoz B, Montecino-Rodriguez E, Signer RAJ, and Dorshkind K. 2012. Blood. 199:5715-5721. (Flow cytometry)

Wang D, Qin H, Du W, Shen Y-W, Lee W-H, Riggs AD, and Liu C-P. 2012. Proc. Natl. Acad. Sci. 109:9493-9498. (in vitro induction of apoptosis)

O’Brian RL, Taylor MA, Hartley J, Nuhsbaum T, Dugan S, Lahmers K, Aydintug MK, Wands JM, Roark CL, and Born WK. 2009. Invest. Ophthalmol. Vis. Sci. 50: 3266-3274. (Immunofluorescence microscopy – OCT embedded frozen tissue)

Matei IR, Gladdy RA, Nutter LMJ, Canty A, Guidos CJ, and Danska JS. 2007. Blood. 109:1887-1896. (Immunoprecipitation)

Harada N, Shimada M, Okano S, Suehiro T, Soejima Y, Tomita Y, and Maehara Y. 2004. J. Immunol. 173:6635-6644. (in vivo T cell depletion)

Kubo RT, Born W, Kappler JW, Marrack P, and Pigeon M. 1989. J. Immunol. 142: 2736-2742. (Origination of clone, Immunoprecipitation, in vitro activation)

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Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.