Customer Service: (855) 848-6626 | 7:30 a.m.-5:00 p.m. PT
Free shipping on orders over $500 View Details

FastPROBE™ 1-Step RT-qPCR Hi-ROX Kit

Sizes available: 100 rxns, 300 rxns
Applications: PCR
SKU: 31-5212
FastPROBE™ 1-Step RT-qPCR Hi-ROX Kit is available for purchase in increments of 1
Product Name Price Qty
FastPROBE™ 1-Step RT-qPCR Hi-ROX Kit ….. Cat.No. 31-5212-0100R ….. Size 100 rxns
FastPROBE™ 1-Step RT-qPCR Hi-ROX Kit ….. Cat.No. 31-5212-0300R ….. Size 300 rxns



FastPROBE™ 1-Step RT-qPCR Hi-ROX Kit contains everything required for efficient cDNA synthesis and probe-based qPCR in a single tube using real-time instruments that support normalization with a standard ROX passive reference dye at a final concentration of 500 nM. This approach offers simplicity and convenience and minimizes the possibility for contamination. An integral component, the Classic++™ Hot Start Taq DNA Polymerase is compatible with all fluorogenic probes including hydrolysis and displacement probes such as TaqMan®, Scorpions® and Molecular Beacons probes. Novel hot start technology prevents primer dimer formation and non-specific amplification resulting in higher yields and specificity. 1-Step Kits include a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Our M-MLV RT is ultra-stable and highly active, working on varied and difficult templates. Convenient and reliable, Tonbo’s FastPROBE 1-Step RT-qPCR Hi-ROX Kit is ideal for the sensitive and efficient quantification of RNA templates, including low copy number targets, under standard or fast cycling conditions. Use for for low copy number detection, microarray validation, SNP genotyping and multiplexing. Please refer to our Instrument Compatibility Guide to ensure you have the correct ROX concentration for your system.

Additional Info

Additional Info

Name FastPROBE™ 1-Step RT-qPCR Hi-ROX Kit
Cat. No. 31-5212
Technical Data Sheet Download TDS
Alternative Names
Application PCR
Need another format or a larger size? Inquire Here

Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHCF = Immunohistochemistry, Frozen Tissue; IHCP = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.