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CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit

Sizes available: 100 rxns, 300 rxns
Applications: PCR
SKU: 31-5202
CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit is available for purchase in increments of 1
Product Name Price Qty
CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit ….. Cat.No. 31-5202-0100R ….. Size 100 rxns
CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit ….. Cat.No. 31-5202-0300R ….. Size 300 rxns



CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit contains everything required for efficient cDNA synthesis and qPCR in a single tube using real-time instruments that support normalization with a standard ROX passive reference dye at a final concentration of 50 nM. This approach offers simplicity and convenience and minimizes the possibility for contamination. Tonbo’s proprietary CYBRFast™ GREEN DNA intercalating dye does not inhibit PCR, resulting in better fluorescent signals than other dyes, and is optimized for use with CYBRFast™ Hot Start Taq DNA Polymerase. Novel hot start technology prevents primer dimer formation and non-specific amplification resulting in higher yields and specificity. 1-Step Kits include a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Our M-MLV RT is ultra-stable and highly active, working on varied and difficult templates. Convenient and reliable, Tonbo’s CYBRFast 1-Step RT-qPCR Hi-ROX Kit is ideal for the sensitive and efficient quantification of RNA templates, including low copy number targets, under standard or fast cycling conditions. Please refer to our Instrument Compatibility Guide to ensure you have the correct ROX concentration for your system.

Additional Info

Additional Info

Name CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit
Cat. No. 31-5202
Technical Data Sheet Download TDS
Alternative Names
Application PCR
Need another format or a larger size? Inquire Here

Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHCF = Immunohistochemistry, Frozen Tissue; IHCP = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.