Customer Service: (855) 848-6626 | 7:30 a.m.-5:00 p.m. PT
Free shipping on orders over $500 View Details

Classic++™ 1-Step RT-PCR Kit

Sizes available: 50 rxns, 100 rxns
Applications: PCR
SKU: 31-5220
Classic++™ 1-Step RT-PCR Kit is available for purchase in increments of 1
Product Name Price Qty
Classic++™ 1-Step RT-PCR Kit ….. Cat.No. 31-5220-0050R ….. Size 50 rxns
Classic++™ 1-Step RT-PCR Kit ….. Cat.No. 31-5220-0100R ….. Size 100 rxns



The Classic++™ 1-Step RT-PCR Kit’s advanced reagents allow for efficient cDNA synthesis and PCR in a single tube. The kit contains a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, a mix that is both thermostable and extremely active. This RTase mix synthesizes cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. For the PCR reaction, Classic++™ Hot Start Taq DNA Polymerase is included. Novel hot start technology improves yields by minimizing or eliminating primer dimer formation and non-specific amplification. Our convenient RT-PCR kit formulation allows for high speed, accurate and reproducible cDNA synthesis and subsequent PCR amplification in a single tube, starting from a variety of target RNA templates.

Additional Info

Additional Info

Name Classic++™ 1-Step RT-PCR Kit
Cat. No. 31-5220
Technical Data Sheet Download TDS
Alternative Names
Application PCR
Need another format or a larger size? Inquire Here

Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHCF = Immunohistochemistry, Frozen Tissue; IHCP = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.