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Gibson Assembly® Site-Directed Mutagenesis

Incorporate up to 5 different site-specific mutations in a single round of mutagenesis and cloning without the need for compatible restriction digestion sites. This flexible site directed mutagenesis kit is compatible with fragments from 500 bp to 5.5 kb and allows single base changes, insertions of up to 40 bp, and deletions of any size.

The Gibson Assembly® Site Directed Mutagenesis (SDM) process begins by creating PCR primers for substitution, insertion, or deletion mutagenesis. PCR is used to introduce the mutations and create overlapping ends. Next, the Gibson Assembly® process is performed to generate double stranded, fully ligated DNA constructs ready for downstream analysis.

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