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Gibson Assembly® HiFi

Gibson Assembly® HiFi 1-Step master mix consists of a blend of enzymes for the seamless joining of 1-5 inserts into a plasmid vector. This single tube, isothermal reaction is compatible with fragments from 500 bp to 32 kb and can be completed in 60 minutes or less. Unlike other approaches, SGI-DNA’s Gibson Assembly® process utilizes Phusion® High-Fidelity DNA polymerase for 3’ end extension. This proof-reading process results in higher numbers of seamless error-free clones ready for multiple downstream applications.

The Gibson Assembly® HiFi 1-Step Kit is available as either the original 2X master mix or 4X high concentration (HC) master mix. The 4X Gibson Assembly® HiFi HC 1-Step kit requires only 2.5 µL of mix per 10 µL reaction making it ideal for assemblies using less concentrated DNA samples. Using either format, multiple DNA fragments can be joined with greater than 90% cloning efficiency.

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4 Item(s)

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