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Gibson Assembly®

Tonbo offers several Gibson Assembly® cloning kits for DNA assembly. Each kit contains specific master mix reagents and proof-reading polymerase.

  Gibson Assembly® HiFi 1-Step Kit
Gibson Assembly® HiFi 1-Step Kit
Gibson Assembly® Ultra Kit
Gibson Assembly® Ultra Kit
Gibson Assembly® Site-Directed Mutagenesis Kit
Gibson Assembly® Site-Directed Mutagenesis Kit
Hands-on time <5 min <6 min <40 min
Total reaction time 60 min 80 min ~4-6 hr
Number of steps 1 2 3
Fragment size range 500 bp to 32 kb 100 bp to 100 kb 500 bp to 5.5 kb
Cloning efficiency >90% >95% 95% single site
Fragments per reaction up to 5 up to 15 up to 5
Maximum construct size (multiple-stage reactions) 100 kb 1000 kb 25 kb



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