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redFluor™ 710 Anti-Human CD45 (HI30)

Sizes available: 25 tests, 100 tests

Applications: FC
SKU: 80-0459
redFluor™ 710 Anti-Human CD45 (HI30) is available for purchase in increments of 1
Product Name Price Qty
redFluor™ 710 Anti-Human CD45 (HI30) ..... Cat.No. 80-0459-T025 ..... Size 25 tests
redFluor™ 710 Anti-Human CD45 (HI30) ..... Cat.No. 80-0459-T100 ..... Size 100 tests



The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.

The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.

Additional Info

Additional Info

Name redFluor™ 710 Anti-Human CD45 (HI30)
Cat. No. 80-0459
Technical Data Sheet Download TDS
Alternative Names Leukocyte Common Antigen, LCA, Ly-5
Gene ID 5788
Clone HI30
Isotype Mouse IgG1, kappa
Reactivity Human
Cross Reactivity Chimpanzee
Format redFluor™
Application Flow Cytometry

Strowig T, Rongvaux A, Rathinam C, Takizawa H, Borsotti C, Philbrick W, Eynon EE, Manz MG, and Flavell RA. 2011. Proc. Natl. Acad. Sci. 108: 13218-13223. (Flow Cytometry)

Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (in vitro blocking, Western Blot)

Zhang M and Varki A. 2004. Glycobiology. 14: 939-949. (Immunoprecipitation)

Gelbmann CM, Leeb SN, Vogl D, Maendel M, Herfarth H, Scholmerich J, Falk W, and Rogler G. 2003. Gut. 52:1448-1456. (Immunocytochemistry)

Yamada T, Zhu D, Saxon A, and Zhang K. 2002. J. Biol. Chem. 277(32): 28830-28835. (in vitro blocking)

Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)

Goto E, Kohrogi H, Hirata N, Tsumori K, Hirosako S, Hamamoto J, Fujii K, Kawano O, and Ando M. 2000. Am. J. Respir. Cell Mol. Biol. 22: 405. (Immunohistochemistry – frozen tissue)

Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)

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Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHCF = Immunohistochemistry, Frozen Tissue; IHCP = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.