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Purified Anti-Mouse CD117 (c-Kit) (ACK2)

Sizes available: 100 µg

Bulk Sizes: 1mg, 25mg, 50mg

*Bulk sizes have a standard lead time of 2 weeks. Customer will be notified if lead time exceeds 2 weeks.

Applications: FC, IHCP, ICC, WB
SKU: 70-1172
Product Name Price Qty
Purified Anti-Mouse CD117 (c-Kit) (ACK2) ..... Cat.No. 70-1172-U100 ..... Size 100 µg
Bulk Purified Anti-Mouse CD117 (c-Kit) (ACK2) ..... Cat.No. 70-1172-B001 ..... Size 1 mg
Bulk Purified Anti-Mouse CD117 (c-Kit) (ACK2) ..... Cat.No. 70-1172-B025 ..... Size 25 mg
Bulk Purified Anti-Mouse CD117 (c-Kit) (ACK2) ..... Cat.No. 70-1172-B050 ..... Size 50 mg



The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.

The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo (use format appropriate for functional assays).

Additional Info

Additional Info

Name Purified Anti-Mouse CD117 (c-Kit) (ACK2)
Cat. No. 70-1172
Technical Data Sheet Download TDS
Alternative Names Steel Factor Receptor, SCFR, cKit
Gene ID 16590
Clone ACK2
Isotype Rat IgG2b, kappa
Reactivity Mouse
Cross Reactivity Rat
Format Purified
Application Flow Cytometry, ICC, IHCP, Western Blot

Tang X, Tian L, Esteso G, Choi S-C, Barrow AD, Colonna M, Borrego F, and Coligan JE. 2012. J. Immunol. 188: 548-558. (Flow cytometry)

Launay J-M, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Cote F, Humbert M, and Maroteaux L. 2012. Blood. 119: 1772-1780. (Immunohistochemistry - formaldehyde fixed tissue)

Mark-Kappeler CJ, Sen N, Lukefahr A, McKee L, Sipes IG, Konhilas J, and Hoyer PB. 2011. Biol. Reprod. 85: 755-762. (in vitro blocking, Western blot – Fischer 344 Rat)

Kim M-H, Granick JL, Wkok C, Walker NJ, Borjesson DL, Curry F-RE, Miller LS, and Simon SI. 2011. Blood. 117:3343-3352. (in vivo depletion)

Fiorina P, Jurewicz M, Vergani A, Petrelli A, Carvello M, D’Addio F et al. 2011. J. Immunol. 186:121-131. (in vivo blocking)

Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, Ordog T, and Farrugia G. 2011. 301: G1044-G1051. (Immunocytochemistry - acetone fixed cells)

Carlsson IB, Laitinen MPE, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RML, and Hovatta O. 2006. Reproduction. 131: 641-649. (Immunohistochemistry – paraffin embedded tissue, in vivo blocking)

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Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHCF = Immunohistochemistry, Frozen Tissue; IHCP = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.