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PerCP-Cyanine5.5 Anti-Human CD4 (RPA-T4)

Sizes available: 25 tests, 100 tests
Applications: FC
SKU: 65-0049
Product Name Price Qty
PerCP-Cyanine5.5 Anti-Human CD4 (RPA-T4) ..... Cat.No. 65-0049-T025 ..... Size 25 tests
$56.00
PerCP-Cyanine5.5 Anti-Human CD4 (RPA-T4) ..... Cat.No. 65-0049-T100 ..... Size 100 tests
$110.00

Description

Details

The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Additional Info

Additional Info

Name PerCP-Cyanine5.5 Anti-Human CD4 (RPA-T4)
Cat. No. 65-0049
Technical Data Sheet Download TDS
Alternative Names Leu-3, T4
Gene ID 920
Clone RPA-T4
Isotype Mouse IgG1, kappa
Reactivity Human
Cross Reactivity Chimpanzee
Format PerCP-Cyanine5.5
Applications Flow Cytometry
Citations*

Toma J, Weinheimer SP, Stawiski E, Whitcomb JM, Lewis ST, Petropoulos CJ, and Huang W. 2011. J. Virol. 85: 3872-3880. (Blocking: HIV-1 interaction)

Porter KA, Kelley LN, Nekorchuk MD, Jones JH, Hahn AB, de Noronha CMC, Harton JA, and Duus KM. 2010. J. Immunol. 185:6480-6488. (Blocking: HIV-1 interaction)

Hsieh S-C, Tsai W-Y, and Wang W-K. 2010. J. Virol. 84(9)

: 4782-4797. (Immunoprecipitation – transfected cells)

Chen X, Wang X, Besra GS, and Gumperz JE. 2007. J. Leukoc. Biol. 82:1455-1465. (in vitro activation)

Thedrez A, de Lalla C, Allain S, Zaccagnino L, et al. 2007. Blood. 110:251-258 (in vitro blocking)

Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1)

:79-87. (Immunohistochemistry – frozen tissue)

Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654. (Immunohistochemistry – OCT embedded frozen tissue)

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Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.