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In Vivo Ready™ Anti-Mouse IL-4 (11B11)

Sizes available: 100 µg, 500 µg

Applications: FC, FA, IHCF, IF, ELISA
SKU: 40-7041
Product Name Price Qty
In Vivo Ready™ Anti-Mouse IL-4 (11B11) ..... Cat.No. 40-7041-U100 ..... Size 100 µg
$118.00
In Vivo Ready™ Anti-Mouse IL-4 (11B11) ..... Cat.No. 40-7041-U500 ..... Size 500 µg
$157.00

Description

Details

The 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14 kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).

The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions. Please choose the appropriate format for each application.

Recent Publications:
Onizawa M, Oshima S, Schulze-Topphoff U, Oses-Prieto JA, Lu T, Tavares R, Produhomme T, Duong B, Whang MI, Advincula R, Agelidis A, et al. 2015. Nat Immunol. 16(6): 618-627. (in vitro activation)

Additional Info

Additional Info

Name In Vivo Ready™ Anti-Mouse IL-4 (11B11)
Cat. No. 40-7041
Technical Data Sheet Download TDS
Alternative Names IL4, Interleukin-4, BSF-1 , nale, na/le, leaf, ultra leaf, ultra-leaf, functional grade
Gene ID 16189
Clone 11B11
Isotype Rat IgG1, kappa
Reactivity Mouse
Cross Reactivity
Format In Vivo Ready™
Applications Flow Cytometry, Functional Assays, IF, IHCF
Citations*

Cook PC, Jones LH, Jenkins SJ, Wynn TA, Allen JE, and MacDonald AS. 2012. Proc. Natl. Acad. Sci. 109: 9977-9982. (in vivo blocking)

Altin JA, Goodnow CC, and Cook MC. 2012. J. Immunol. 5478-5488. (Flow cytometry)

Tofukuji S, Kuwahara M, Suzuki J, Ohara O, Nakayama T, and Yamashita M. 2012. J. Immunol. 188: 4846-4857. (in vitro Th1 polarization)

Weber KS, Hildner K, Murphy KM and Allen PM. 2010 J. Immunol. 185: 2836-2846 (in vitro Th1 polarization, ELISA)

Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy – frozen tissue, Immunohistochemistry – frozen tissue)

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Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.