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In Vivo Ready™ Anti-Mouse IL-12/IL-23 p40 (C17.8)

Sizes available: 100 µg, 500 µg

Applications: FC, FA, IF, IP, WB, ELISA
SKU: 40-7123
Product Name Price Qty
In Vivo Ready™ Anti-Mouse IL-12/IL-23 p40 (C17.8) ..... Cat.No. 40-7123-U100 ..... Size 100 µg
$132.00
In Vivo Ready™ Anti-Mouse IL-12/IL-23 p40 (C17.8) ..... Cat.No. 40-7123-U500 ..... Size 500 µg
$167.00

Description

Details

The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, macrophages, and dendritic cells, and has been shown to target naïve, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19; its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the receptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.

As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine. Please choose the appropriate format for each application.

Additional Info

Additional Info

Name In Vivo Ready™ Anti-Mouse IL-12/IL-23 p40 (C17.8)
Cat. No. 40-7123
Technical Data Sheet Download TDS
Alternative Names Interleukin-12, IL12 / Interleukin-23, IL23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Gene ID 16160
Clone C17.8
Isotype Rat IgG2a, kappa
Reactivity Mouse
Cross Reactivity
Format In Vivo Ready™
Applications Flow Cytometry, Functional Assays, IF, IP, Western Blot
Citations*

Dong H, Franklin NA, Roberts DJ, Yagita H, Glennie MJ and Bullock TNJ. 2012. J. Immunol. 188: 3829-3838. (in vivo blocking)

Prabhakara R, Harro JM, Leid JG, Keegan AD, Prior ML, and Shirtliff ME. 2011. Infect. Immun. 79: 5010-5018. (in vivo blocking)

Chmielewski M, Kopecky C, Hombach AA, and Abken H. 2011. Cancer Res. 71: 5697-5706. (ELISA)

Lo C-H, Lee S-C, Wu P-Y, Pan W-Y et al. 2003. J. Immunol. 171: 600-607. (Immunoprecipitation)

Belladonna ML, Renauld J-C, Bianchi R, Vacca C, Fallarino F, Orabona C, Fioretti MC, Grohmann, and Puccetti P. 2002. J. Immunol. 168: 5448-5454. (Western Blot)

Ludviksson BR, Ehrhardt RO, and Strober W. 1999. J. Immunol. 163: 4349-4359. (Immunofluorescence microscopy – frozen tissue)

Kato K, Shimozato O, Hoshi K, Wakimoto H, Hamada H, Yagita H, and Okumura K. 1996. Proc. Natl. Acad. Sci. 93:9085-9089. (Immunoprecipitation; ELISA)

Wysocka M, Kubin M, Vieira LQ, Ozmen L, Garotta G, Scott P and Trinchieri G. 1995. Eur. J. Immunol. 25: 672-676. (Origination of clone; Immunoprecipitation, Western Blot)

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Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.