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Ghost Dye™ Violet 510

Sizes available: 100 tests, 500 tests

Applications: FC
SKU: 13-0870
Ghost Dye™ Violet 510 is available for purchase in increments of 1
Product Name Price Qty
Ghost Dye™ Violet 510 ..... Cat.No. 13-0870-T100 ..... Size 100 tests
$44.00
Ghost Dye™ Violet 510 ..... Cat.No. 13-0870-T500 ..... Size 500 tests
$170.00

Description

Details

Ghost Dye™ Violet 510 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 510 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

I recently obtained an aliquot of Ghost Dye 510 from my core flow lab. I titrated the reagent against a mixture of heat-killed and untreated PBMC. Spooooky Bright! I will be replacing my current Live/Dead indicator with the Ghost Dye 510.

Cindy L. Zuleger, Ph.D.
University of Wisconsin

Recent Publications:
Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Flow Cytometry)

Lee RA, Mao C, Vo H, Gao W and Zhong X. 2015. Ann NY Acad Sci. doi: 10.1111/nyas.12865. (Flow Cytometry)

Watkin LB, Jessen B, Wiszniewski W, Vece TJ, Jan M, Sha Y, Thamsen M, Santos-Cortez RL et al. 2015. Nat Genet. 47: 654-660. (Flow Cytometry)

Vo H, Chiu J, Allaimo D, Mao C, Wang Y, Gong Y, Ow H, Porter T and Zhong X. 2014. Immun, Inflamm & Dis. 2(4): 254-261. (Flow Cytometry)

Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)

Additional Info

Additional Info

Name Ghost Dye™ Violet 510
Cat. No. 13-0870
Technical Data Sheet Download TDS
Protocol Download Protocol
Excitation Laser Violet (405 nm)
Emission (nm) 510
Formulation 1 µL/test in DMSO
Applications Flow Cytometry
Need Another Format? Inquire Here

Applications Guide:
FC = Flow Cytometry, FA = Functional Assays, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IHCF = Immunohistochemistry, Frozen Tissue, IHCP = Immunohistochemistry, Paraffin-embedded Tissue, IF = Immunofluorescence Microscopy, IP = Immunoprecipitation, WB = Western Blot, EM = electron microscopy


*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.