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Buffers and Solutions

Tonbo Biosciences is proud to provide your favorite buffers and solutions for flow cytometry, manufactured to deliver consistent, accurate results. Our Foxp3 / Transcription Factor Fix/Perm Solutions are formulated for optimal resolution and low background in your analysis of key nuclear proteins such as Foxp3 and ROR gamma.

Foxp3 / Transcription Factor Staining Buffer Kit – Analysis of Mouse Foxp3

Foxp3 / Transcription Factor Staining Buffer Kit – Analysis of Mouse Foxp3

Get the same great results for less!
C57Bl/6 splenocytes were stained with FITC Anti-Mouse CD4 followed by intracellular staining with APC Anti-Mouse Foxp3 (clone 3G3). Intracellular staining was performed using Foxp3 / Transcription Factor Staining Buffer Kit (TNB-0607-KIT) manufactured by Tonbo Biosciences (right panel), or the comparable kit available from BD Biosciences (left panel), and eBioscience (middle panel).

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  • Cell Stimulation Cocktail (500X)

    Sizes available: 100 µL
    Applications: FC, FA
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    Starting at: $59.00

  • Flow Cytometry Perm Buffer (10X)

    Tonbo Biosciences Flow Cytometry Perm Buffer (10X) is provided as a concentrate which, when diluted with distilled water to a 1X solution, provides best results in intracellular staining protocols for cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and wash steps. The Flow Cytometry Perm Buffer is formulated for optimal resolution and low background when used in fluorophore-labeled antibody staining protocols, followed by flow cytometric analysis.

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    Starting at: $63.00

  • Foxp3 / Transcription Factor Fix/Perm Concentrate (4X)

    Tonbo Biosciences Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) is a concentrated solution which, when diluted with Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (cat. no. TNB-1022-L160), provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies, followed by flow cytometric analysis. These reagents are formulated for optimal resolution and low background in your analysis of key nuclear proteins such as Foxp3.

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    Starting at: $63.00

  • Foxp3 / Transcription Factor Fix/Perm Diluent (1X)

    Tonbo Biosciences Foxp3 / Transcription Factor Fix/Perm Diluent (1X), is intended for use with Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) (cat. no. TNB-1020-L050), for best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies, followed by flow cytometric analysis. These reagents are formulated for optimal resolution and low background in your analysis of key nuclear proteins such as Foxp3.

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    Starting at: $24.00

  • Foxp3 / Transcription Factor Staining Buffer Kit

    Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains the following components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma. Learn More

    Starting at: $118.00

  • Lymphocyte Separation Medium

    Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood. The use of LSM is based on the method of isolating lymphocytes using density gradient centrifugation (differential centrifugation), resulting in the formation of several distinct cell layers. Mononuclear cells (lymphocytes and monocytes) and platelets will be contained in the banded plasma-LSM interphase due to their density. Erythrocytes and granulocytes will migrate through the gradient to the bottom of the tube. Lymphocytes can be recovered by aspirating the plasma layer and removing the cells.

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    Starting at: $39.00

  • RBC Lysis Buffer (10X)

    RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent. The diluted 1X working solution will lyse red blood cells in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer (10X) does not contain a fixative so the cells remain viable after red blood cell lysis. Learn More

    Starting at: $36.00

  • Sheath Fluid (30X)

    Tonbo Biosciences Sheath Fluid (30X) is a pre-filtered, pH balanced phosphate buffered saline solution that can be used for transporting particles though any flow cytometer. This concentrated formula is easily prepared, uses less storage space and costs less to ship than conventional 20 L containers. Each bottle provides sufficient concentrate to prepare 20 liters of 1x sheath fluid. Learn More

    Starting at: $29.00

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